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Effects of Concentrated Solutes on Cellular Membranes & Water Potential. Statement of the Problem: How does diffusion across the who is to blame death of romeo essay, cell membrane work? What molecules pass through the cell membrane easier than others? Is there any influence of solute concentration to the net movement of water molecules across the cell membrane? What is the effect of water potential on the cell membrane? Cell membranes act as a barrier for the cell. Solved Case Finance. It keeps together enzymes, DNA, and pathways for metabolic reactions. Cell membranes dispose of waste products from the cell and lets important molecules, like water and who is for the and juliet conclusion oxygen, into essay competition, the cell. The membrane is semipermeable, meaning only specific molecule may enter the cell. The passing of molecules is either through active transport (passage of materials using energy) or passive transport (passage of materials using kinetic energy). Molecules are in constant, random motion (Brownian motion) and if they collide with the to blame for the death of romeo, membrane, they will rebound. If the molecules are headed toward an open pore in the cell membrane, it may pass through the pore or rebound depending on its size to the pore. The passage of solved studies for mba, molecules across the cell membrane from an area of high concentration to low concentration is call diffusion. The diffusion of water molecules across the for the death and juliet essay conclusion, cell membrane is called osmosis. The Hallmark Of The Personal Essay. Water is isotonic and moves freely across the cell membrane and helps maintain its fluid mosaic model characteristic. Hypertonic solutions are solutions with higher amounts of solutes and hypotonic solutions are solutions with a lower amount of solutes. The movement of water across the cell membrane depends on the concentration of solutes on both sides of the cell membrane. When water moves out of the cell, the cell will shrink, and when water moves into the cell, the cell will swell and possibly burst. Who Is For The. Cell walls are present in plant cells which prevent the cell from bursting once it swells. When water enters the plant cell, the membrane is pressed up against the cell wall and creates turgor pressure. Water potential is review used to sum up the differences in who is to blame for the death of romeo and juliet conclusion solute concentration and pressure to predict the direction water will diffuse in living plant tissues. Water potential is germinal essay measured in bars, metric units of pressure equal to 10 newtons per cm 2 or 1 atmosphere. It is also abbreviated by the Greek letter “psi” (Ψ). Two major factors of water potential are solute potential (Ψs), the dependent on solute concentration, and pressure potential (Ψp), which represents exertion of pressure on a solution in positive or negative. The formula for water potential is: Water Potential (Ψ) = Pressure Potential (Ψp) + Solute Potential (Ψs). Pure water has a water potential of 1 atmosphere. Dissolving substances in water will result the water potential dropping below zero. When solute concentration increases, water potential decreases. Pressure potential may be positive, negative, or zero. Even though water is who is of romeo and juliet conclusion diffused in all directions, water will always diffuse from an area of solved case studies for mba finance, high water potential to and area of low water potential. In terms for the potato, the system is who is death of romeo essay conclusion open to the atmosphere, so Ψp = 0. Germinal Essay. A value of who is for the death of romeo, -9 will be assigned for variable Ψs. Pure water is Ψ = 0 + 0 = 0 when the potato cell is Ψ = 0 + (-9) = -9. At equilibrium, pure water is Ψ = 0 + 0 = 0 and the potato cell is Ψ = 9 + (-9) = 0. If we add a solute to the water, then the water plus solute is Ψ = 0 + (-15) = -15 and the potato cell is Ψ = 0 + (-9) = -9. Who Is To Blame Of Romeo And Juliet Essay. The process of the for the and juliet essay, cell wall pulling away from the cell membrane in a plant cell is called plasmolysis. If we determine the molarity of the sucrose solution that will help produce equilibrium between the solution and the contents of the potato cell, we can determine solute potential: Ψs – iCRT, where i is ionization constant, C is molar concentration of sucrose per liter at of the personal essay is its equilibrium, R is for the of romeo and juliet essay conclusion pressure constant (0.0831 liter bar/mole K), and T is temperature of solution in Kelvins. Activity A: Diffusion. Hypothesis: If we add glucose-starch solution to a dialysis tubing bag and submerged it in a cup of distilled water and IKI solution, then glucose will leave the dialysis tubing bag through pores into the IKI solution through diffusion. 12 inch Strip of Dialysis Tubing. 15 mL of 15% Glucose/1% Starch Solution. 200 mL of master's literature, Distilled Water. 30 mL of Iodine-Potassium Iodide (IKI) Solution. 4 Glucose Test Strips. First, we poured 160-170 mL of distilled water into who is to blame and juliet essay, a cup and added about 4 mL of IKI solution to the water and mixed well. We recorded the germinal essay, initial color of the who is for the of romeo and juliet essay conclusion, solution in Table 1. Next, we dipped a glucose test strip into the solution and recorded the initial glucose test results of the solution in Table 1 using a + symbol to indicate a positive result for glucose and a – for a negative result for glucose. After we were finished, we discarded the used glucose test strip. Next, we dipped a new glucose test strip into the glucose/starch solution. We recorded the initial glucose test result in Table 1. Then we discarded the who is for the and juliet essay, used glucose test strip. After soaking a piece of dialysis tubing in to blame death of romeo and juliet essay water, a group member rolled the the hallmark, tubing between their thumb and who is to blame for the death essay conclusion index finger to open it. We tied one end of the dialysis tubing to create a bag. Using a small funnel, we poured 15 mL of the glucose/starch solution into the dialysis bag. Then we tied off the top of the bag to close it while leaving enough room in competition the bag for expansion. We recorded the initial color of the glucose/starch solution in Table 1. We then placed the dialysis bag into the solution in the cup. In doing so, we made sure the who is to blame for the death of romeo, entire bag was covered by the solution in the cup. We then waited 30 minutes and worked on an activity relating to solved case, Figure 2. Figure 2 Activity: We were to indicate initial locations of molecules and who is for the death essay predict in which direction they would move in diffusion (into the bag, out of the bag, both into and out of the bag, or none). Case Studies Finance. After completing the activity with figure 2, we were able to compare our predictions about the outcome to the actual results of the experiment. Distilled water was initially in the cup and is predicted to stay in the cup. IKI was initially in the cup and is predicted to stay in the cup and also move into the dialysis bag. Glucose was initially in the dialysis bag and is predicted to flow in who is to blame for the of romeo and out of the dialysis bag and exist in both the germinal essay, cup and dialysis bag. Sucrose was initially in the dialysis bag and for the death of romeo essay conclusion is predicted to stay in the dialysis bag. After 30 minutes, we removed the dialysis bag from the who is to blame for the death and juliet, cup and dried it with a paper towel. We then cut a hole into the bag large enough for a glucose test strip to who is for the death of romeo conclusion, enter. We then collected the final amounts of glucose and completed Table 1. Glucose Test Results. When comparing our results to our predictions, we had predicted correctly. We had no conflicts that would have made us revise our predictions. The results show the locations each molecule had gone and master's literature were the molecules ended up and proved our predictions were correct. This activity proved the for the of romeo and juliet conclusion, net movement of finance essay, glucose from the dialysis bag to who is to blame death essay conclusion, the cup and both the cup and dialysis tested positive for glucose at the end of the experiment. Without Essays. Distilled water was staying in the cup and IKI stayed in the cup but also moved into the dialysis bag because the resulting color was dark blue/black (when IKI reacts with starch, it turns dark blue/black). The data in this experiment tells us the sizes of molecules would have to be small enough to fit through the dialysis tubing because if it is not small enough, then the who is to blame for the and juliet essay conclusion, tubing will rebound the molecules and not let them pass through. Activity B: Osmosis. Hypothesis: If we add higher concentrations of germinal essay, sucrose to the dialysis bag, then the net movement of water into the dialysis bag will increase. 6 12 inch Dialysis Tubing Strips. 30 mL of Distilled Water. 30 mL of 0.2 M Sucrose Solution. 30 mL of who is death essay conclusion, 0.4 M Sucrose Solution. 30 mL of 0.6 M Sucrose Solution. 30 mL of 0.8 M Sucrose Solution. 30 mL of 1.0 M Sucrose Solution. For each sucrose solution, we poured 160-170 mL of distilled water into a cup. We then labeled the cup with the concentration of sucrose that we tested. A group member then took a piece of dialysis tubing and opened it by of romeo conclusion, rolling it between their thumb and index finger after being soaked in water. Then we tied off one end of the dialysis tubing to to blame and juliet conclusion, create a bag. Using a funnel, we poured 25 mL of sucrose solution into the dialysis bag. Leaving enough room in the bag for the hallmark of the personal expansion, we tied off the bag and dried it will paper towels. We weighed the who is to blame for the, bag and recorded its initial mass in Table 2. After measuring initial mass, we placed the finance, bag in the cup of water, making sure the death of romeo, bag was completely submerged in the water. We waited for 30 minutes before continuing. After 30 minutes, we removed the bag from the water and scholarships for college essays dried it with paper towels. We then weighed the bag and recorded the final mass in Table 2. Contents in the Dialysis Bag. Percent Change in Mass. 0.0 Sucrose (Distilled Water) To calculate the percent change in mass, we used the formula: % Change in Mass = Change in Mass/Initial Mass x 100% The change in mass in this activity indicates whether or not a solution entered or left the dialysis bags during the experiment. In the case of Activity B, the change in mass increased and shows water entered the and juliet essay conclusion, bags during the experiment. Our hypothesis for this experiment was, “If we add higher concentrations of to blame for the, sucrose to the dialysis bag, then the net movement of who is to blame of romeo and juliet essay conclusion, water into the dialysis bag will increase”. In this experiment, the variable being tested is water. Osmosis is the diffusion of water from a high concentration to a low concentration and water was the variable being tested in this activity because it is what made the mass increase for every sucrose solution. The amount of sucrose solution, dialysis bag, and germinal essay time could all influence the outcome of who is to blame of romeo essay, this experiment. The higher the amount of sucrose causes for more water to move into the dialysis bag. The dialysis bag is an influence because of pore size in relation to the size of the water molecules. Time could influence the movement of water because maybe, with more time, more water will move into the dialysis bag than shorter time periods. When comparing our group’s data and graph to competition, other group’s data and for the death and juliet graphs, we all had collected similar data that was not exact but precise. The graph we made is an accurate representation of our data and how the the hallmark of the personal essay is its, mass changed due to the sucrose solutions because water was adding to the weight of the dialysis bag. In osmosis, water molecules moved into for the death of romeo conclusion, the dialysis bags with higher sucrose molarities. The solutions in the bag and germinal essay outside of the who is to blame for the death of romeo conclusion, bag were not isotonic to each other during this experiment because of the change in mass. If the experiment asked for the water to be inside the dialysis bag and the sucrose solution to be inside the cup, then we would have seen the mass of the solved, dialysis bag decrease the higher the molarity of the sucrose. For The Death Of Romeo And Juliet Essay. Drinking seawater can dehydrate the body because the essay, water is hypertonic to the cells lining the small intestine, so this will pull the water out of the cells dehydrating the body. Activity C: Plant Cells and Water Potential. Hypothesis: If we immerse potato cores in sucrose solutions, then the higher molarity solutions will cause the mass of the potatoes to decrease. 120 mL of Distilled Water. 120 mL of 0.2 M Sucrose Solution. 120 mL of 0.4 M Sucrose Solution. 120 mL of 0.6 M Sucrose Solution. 120 mL of 0.8 M Sucrose Solution. 120 mL of who is for the death of romeo and juliet essay conclusion, 1.0 M Sucrose Solution. First, we labeled a cup with the concentration of germinal essay, sucrose we were going to test for each concentration of sucrose. Using a scalpel, we cut four cylinders of potato tissue and removed all skin from the cylinders. Then we cut each cylinder into sections around 3 cm long. Using an who is of romeo and juliet essay, electronic balance, we measured the weight of the scholarships without, 6 3cm long potato sections and recorded their initial weight in Table 3. After measuring the weight of all the potato sections, we placed all of the potatoes into the labeled cups. We then poured 100 mL of the assigned sucrose solution into the cup and covered them with plastic wrap. We then allowed for to blame of romeo and juliet conclusion the potatoes to sit in the sucrose solutions overnight. After a 24 hour period, we recorded the ambient temperature given by finance essay competition, the teacher in who is to blame for the death essay conclusion Table 3. Next we removed all potato sections from all sucrose solutions and blotted them with a paper towel to who is death conclusion, remove the excess solution. We then immediately weighed the potatoes and recorded their mass in Table 3. After measuring the weight, we covered the potato sections back up to prevent evaporation of the solution. % Change in Mass (Class Average) 0.0 M Sucrose (Distilled Water) For this graph, we will use C = 0.2 moles of for the and juliet essay, Sucrose/Liter for equilibrium. The temperature of thesis literature review, 23*C was changed to Kelvins by the formula K = C + 273. The ambient temperature for the experiment is changed to 296 Kelvins. For The Death And Juliet. To calculate solute potential at equilibrium, we used the master's literature, formula Ψs = -iCRT. Ψs = (1)(0.2 moles)(0.0831liter bar/mole)(296 K) The water potential of the solution at equilibrium is Ψ = 4.91 + (-4.91) = 0. The water potential for the potato cells at equilibrium is Ψ = 9 + (-9) = 0. When a chef chops vegetables into a bowl of water in problem 6, the for the and juliet conclusion, vegetable slices would gain water because the water will move into the vegetable due to pressure and will cause the vegetable to slightly swell. The farmer using the sea water from the scholarships for college students without, Mediterranean Sea in problem 7 should be advised because the salt water will pull the remaining water out of the wheat plant cells because of its hypertonic nature. When a marine clam is added to a freshwater aquarium in problem 8, the water around it is hypotonic, so the clam will eventually die due to water moving from high concentration to who is for the of romeo and juliet essay, low concentration. In problem 9, carrot cells closer to the sugar would have more water in them as opposed to cells on the outside of the carrot because of the net movement of water toward high molarities of sugars. Scholarships For College Without Essays. Cells on the outside of the carrot will have water but not as much as the middle of the carrot. Also, the amount of water in the cup will be lower because the water will be moving toward the higher concentration of sugar at the middle of the carrot. For this lab all experiments and results for activities A, B, and C accept the hypothesis for each given activity. For activity A, the hypothesis stated that if we added glucose-starch solution to a dialysis tubing bag and submerged it in a cup of distilled water and IKI solution then glucose will leave the dialysis bag through the pore into the IKI and who is to blame death distilled water solution through diffusion. Our data supports our hypothesis because at the end of the experiment, glucose was present in both the cup with the who is to blame death and juliet conclusion, IKI solution and to blame for the and juliet conclusion the dialysis bag. For activity B, the hypothesis stated that if we added higher concentrations of sucrose to the dialysis bag then the finance, net movement of water into the dialysis bag will increase. Our data supports our hypothesis because at the end of the experiment, the data in Table 2 shows higher concentrations of sucrose caused more water to enter into the dialysis bag. This increased the weight of the dialysis bag as well. For activity C, the hypothesis stated that if we immerse potato cores in a sucrose solution, then higher molarity solutions will cause the mass of the potatoes to decrease. Our data supports our hypothesis because at to blame death of romeo and juliet conclusion the end of the experiment, the data in Table 3 shows at higher sucrose solution molarities, the mass of potatoes decreased. Germinal Essay. This means that the net movement of water when toward the who is for the death and juliet essay, sucrose, but instead of going into the object, like in activity B, the water moved out of the object. In this AP lab, I learned more about diffusion and osmosis through the cell membrane and also through organisms and plants. I also learned completely about water potential, what it is, and how it shows where water is moving (whether it is moving in or out of a cell or organism). I learned more about how cell membranes are semipermeable and only allow certain materials in and out of it. Scholarships For College Students Without Essays. Also, I learned how not all materials can fit through the membrane’s pores and are rebounded off of the membrane. I learned more about osmosis and how water passed through the dialysis bag in activity B to the sucrose solution. I learned in activity C about the water potential and net movement of water and how it moves toward higher amounts of sucrose solution more than lower amounts. Errors in this lab could include clogging of the dialysis bag pores with oil from our skin when rubbing the tubing to open it. Also, there could have been human error in calculations, blotting dialysis bags dry, and not leaving enough room in the bags for expansion. Who Is For The Death And Juliet Conclusion. Electronic errors include measuring errors when using an essay competition, electronic balance.

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